DMMC COURSES & WORKSHOPS

TECHNIQUES & STRATEGIES IN MOLECULAR MEDICINE
Mon 8 Dec - Wed 10 Dec 2003; 0900-1300 on Mon & Tue; 0900-1800 on Wed.
Venue: Royal College of Surgeons in Ireland (location)

Designed to give a broad introductory overview of various techniques and their applications

NUCLEIC ACIDS 1 (Mon 8 Dec 2003; 0900-1300; Houston Lecture Theatre)
0900 Differential gene expression: subtractive hybridization, oligonucleotide microarrays, cDNA microarrays (Dr Madeline Murphy, UCD).
0950 Manipulating gene expression 1: antisense, gene therapy (Prof Mark Lawler, TCD).
1040 Coffee/Tea
1110 RNA detection and quantitation: RT-PCR, Real Time PCR, Northerns (Dr John Crean, UCD).
1200 Manipulating gene expression 2: RNAi, cloning strategies (Dr Ross McManus, TCD).

NUCLEIC ACIDS 2 (Tue 9 Dec 2003; 0900-1300; Cheyne Lecture Theatre)
0900 Cytogenetics and genomic microarrays (Dr Ray Stallings, UCD).
0950 Mutation detection, SNP analysis and genetic linkage (Dr Denis Shields, RCSI).
1040 Coffee/Tea
1110 Manipulating gene expression 3: model organisms (Dr Breandan Kennedy, UCD). Follow these links for supplementary material:
a) The WWW Virtual Library: Model Organisms
b) NIH: Model Organisms for Biomedical Research
1200 Manipulating gene expression 4: transgenics (Dr Jane Farrar, TCD).

PROTEINS (Wed 10 Dec 2003; 0900-1300; Houston Lecture Theatre)
0900 Protein expression and purification (Dr Henry Windle, TCD).
0950 Protein-protein interactions: yeast 2-hybrid, FRET (Dr Niamh Moran, RCSI).
1040 Coffee/Tea
1110 Protein arrays in proteomics (Dr Dolores Cahill, RCSI).
1200 Expression proteomics: biomedical applications (Prof. Stephen Pennington, UCD).

CYTOMETRY TECHNIQUES (Wed 10 Dec 2003; 1400-1800; Houston Lecture Theatre)
1400 Immunodetection: Westerns, ELISA (Dr Leonie Young, UCD).
1450 Flow cytometry (Dr Bill Watson, UCD).
1540 Coffee/Tea
1610 Confocal microscopy and real time imaging (Dr Yuri Volkov, TCD).
1700 Laser Capture Microdissection, tissue arrays, in situ hybridisation (Dr Orla Sheils, TCD).