DMMC COURSES & WORKSHOPS

PROTEOMICS: METHODS & APPLICATIONS
2 - 16 Jun 2005; 1330-1730 weekly (Thu)
Venue: Cheyne Lecture Theatre, Royal College of Surgeons in Ireland (location)

The wealth of genomic data now available for a wide variety of organisms provides us with the opportunity to perform comprehensive protein profiling studies of complex biological systems. Application of the science of proteomics will result in new insights into cellular functions in health and disease, and lead to the discovery of new diagnostic agents and therapeutic targets. This course is designed to give an overview of the main workflows that are currently being used for protein profiling:
1) two-dimensional gel electrophoresis
2) liquid chromatography coupled either on- or off-line to mass spectrometry
3) protein and antibody micro-arrays
The applications of these complementary approaches to biomedical research will be discussed. The course is designed to equip participants with the level of knowledge required to assess the potential applicability of proteomics to their research.

Follow the links below to read lecture abstracts and instructor biographies (these appear as soon as we receive them).

Day 1, Thu 2 Jun; 1300-1730
Course Overview & Gel-Based Proteomics
Coordinator: Dr Achim Treumann (RCSI)

1300 Course Overview (see outline above)
         Prof Mike Dunn, UCD
1330 2-D Electrophoresis in Proteomics abstract
         Medical Applications of Gel-based Proteomics
         abstract
         Prof Mike Dunn will now present both topics
         Suggested reading
1510 Coffee/Tea
1550 Biological Mass Spectrometry: Overview
         Dr Achim Treumann, RCSI  abstract  
1640 MALDI-TOF and Peptide Mass Fingerprinting
         Dr Achim Treumann, RCSI 

Day 2, Thu 9 Jun; 1330-1730
In Silico & Quantitative Proteomics
Coordinator: Dr Gerard Cagney (Conway Institute, UCD)

1330 Tandem Mass Spectrometry & Peptide
         Sequencing Dr Achim Treumann, RCSI
1420 In Silico Proteomics Andrew Lyall, CHP, RCSI
1510 Coffee/Tea
1550 Shotgun Proteomics & Quantitative Proteomics
         Dr Gerard Cagney, UCD  abstract
1700 Medical Applications of Liquid Chromatography-
         Based Proteomics Dr Patricia Maguire, UCD
         abstract   Suggested reading

Day 3, Thu 16 Jun; 1330-1730
Array-Based Proteomics
Coordinator: Prof Dolores Cahill (Centre for Human Proteomics, RCSI)

1330 High-throughput Protein Expression
         Prof Dolores Cahill, CHP, RCSI  abstract
1420 Generation of Protein and Antibody Arrays
         Dr Sabine Baars, CHP, RCSI  abstract
1510 Coffee/Tea
1550 Diagnostic & Medical Applications of Protein
         Biochips Prof Dolores Cahill, CHP, RCSI  abstract
1640 Determining Protein-Protein Interactions Using
         Protein Arrays: Relevance to Platelet Biology
         Dr Niamh Moran, RCSI  abstract