DMMC COURSES & WORKSHOPS

MICROARRAY ANALYSIS
15 Mar 2005; 0900-1700
Venue: Conway Institute, University College Dublin (location)

A 1-day course in which presentations on microarray experimental design, data analysis, and interpretation of data are followed by hands-on tutorials.

The whole course (morning lecture and afternoon hands-on sessions) can accommodate a maximum of 50 attendees (who will receive a certificate of attendance). The morning lecture session only is open to additional attendees.

Course Coordinator: Dr John O'Brien (Centre for Human Proteomics, RCSI)

LECTURES (Conway Lecture Theatre; 0900-1230)

Introduction Dr John O'Brien

Project Design
Dr Sandra Austin (UCD)
Including: array types, biological objective, sources of variation, replicates, Type I and Type II errors, factors affecting Power of analysis, Power analysis assumptions.

Normalisation Dr Peadar Ó Gaora (UCD)
Including: sources of variation, normalisation methods, external controls, technology-specific issues, and normalisation software.

Statistics for Array Analysis Dr Patrick Dicker (RCSI)
Covering: terminology, types of variables measured, descriptive and inferential statistics, univariate methods, multivariate methods including clustering and Principle Component Analysis.

The R Package & Bioconductor Dr Aedín Culhane (UCD)
An overview of Bioconductor microarray data analysis software, part of the open source R statistical package.

Interpretation Dr John O'Brien
An overview of resources available for interpretation of microarray data: literature, databases, and gene ontologies.

HANDS-ON TUTORIALS (Conway Computer Lab; 1400-1700)

Tour of NetAffx Dr James McRedmond (UCD)
The Affymetrix NetAffx Analysis Center enables researchers to correlate their GeneChip array results with array design and annotation information.

R Package/Bioconductor Dr Aedin Culhane

Experimental Design Dr Patrick Dicker

Gene Ontology & Other Tools Dr John O'Brien