DMMC Course: UNRAVELLING CHROMATIN & THE ROLE OF EPIGENETICS IN DISEASE

1430-1450 Tuesday 25 April 2006. UCD Conway Institute Lecture Theatre

Optimizing chromatin immunoprecipitation: tips and suggestions
Dr Rachel Imoberdorf, Senior Development Scientist, Abcam

Chromatin immunoprecipitation (ChIP) is the key method used to map the location of modified histones and other chromosomal proteins in the genome. The ChIP assay combines two straightforward steps, first in vivo formaldehyde crosslinking of whole cells that freezes protein-protein-DNA interactions, followed by immunoprecipitation of the protein-DNA complexes with specific antibodies from sonicated extracts. Here we will provide further insights into this powerful method.

For expert protocols and tips visit www.abcam.com/chromatin/chip