DMMC Course: TECHNIQUES & STRATEGIES IN MOLECULAR MEDICINE

0900-0945 Thursday 7 December 2006. Albert Lecture Theatre, RCSI

Protein expression and purification
Dr Henry Windle (Institute of Molecular Medicine, TCD)

This lecture will cover the basics of protein expression and purification. Emphasis will be placed on alternative strategies and issues that should be considered prior to selection of specific expression systems and purification strategies. As protein purification methodologies are generally well described and accessible, only a brief overview of these will be given but with emphasis on common problems that can arise, particularly for those about to attempt purification for the first time. Finally, technical issues associated with downstream applications of purified proteins will be considered. The following books from The Practical Approach series by IRL Press (at OUP) are an invaluable aid with detailed and reliable protocols: Protein Purification Applications; Protein Purification Methods (2001, Editor Simon Roe).