DMMC Course: TECHNIQUES & STRATEGIES IN MOLECULAR MEDICINE

1400-1445 Tuesday 5 December 2006. UCD Conway Institute Lecture Theatre, UCD

Introduction to cloning
Dr Ross McManus (Institute of Molecular Medicine, TCD)

Even in the post genome era, DNA cloning is essential to the manipulation and stable propagation of genetic material. This talk will cover the basic aspects of DNA cloning, ranging from the anatomy of cloning vectors to the choice of vectors based on the cloning strategy employed. The strategy employed will depend on the overall objectives of the project and the nature of the starting information or material available. Thus different choices and approaches would be employed for a sequencing project compared with a genome mapping project. I will discuss a number of basic and specialised cloning strategies to illustrate some of the options and possibilities available.