DMMC Course: TECHNIQUES & STRATEGIES IN MOLECULAR MEDICINE

1540-1630 Wednesday 8 December 2004. Houston Lecture Theatre, RCSI

Transgenics and knockouts Dr Derek Brazil, UCD

This lecture will provide a broad overview of the strategies used to generate both transgenic and knockout mice, starting from the generation of the DNA constructs using cDNAs or genomic DNA, and proceeding through embryonic stem cell biology, to aggregation and chimeric mouse generation. Details on genotyping of transgenic animals, as well as phenotype characterization will be discussed. Specific examples such as the IRS-2 knockout will be cited.