DMMC Course: TECHNIQUES & STRATEGIES IN MOLECULAR MEDICINE

1330-1415 Tuesday 7 December 2004. Albert Lecture Theatre, RCSI

RNA detection and quantitation Dr John Crean, UCD

RNA analysis is the starting point for many molecular biology procedures. This lecture will provide an introduction to RNA procedures such as purifying RNA, assessing the quality of purified RNA, amplifying via RT-PCR, and assessing mRNA levels by procedures including Northern blot and Real-Time (Taqman) PCR.