DMMC Course: TECHNIQUES & STRATEGIES IN MOLECULAR MEDICINE

1415-1500 Friday 10 December 2004. Houston Lecture Theatre, RCSI

Cell imaging and sorting – flow cytometry Dr William Watson, UCD

Flow cytometry is a method for quantitating components or structural features of cells, primarily by optical means. Although it makes measurements on one cell at a time, it can process thousands of cells in a few seconds. Since cell types can be distinguished by quantitating structural features, flow cytometry can be used to count prokaryotic or eukaryotic cells of different types in complex mixtures.

Flow cytometry overview