DMMC Course: TECHNIQUES & STRATEGIES IN MOLECULAR MEDICINE

1610-1650 Thursday 9 December 2004. Houston Lecture Theatre, RCSI

Protein arrays: applications in proteomics and beyond
Prof Dolores Cahill, CHP, RCSI

Developing and applying high density arraying technologies and automation to generate high content, high-density protein arrays and antibody arrays will be described. One of the main focuses is to provide the protein content to put on the arrays, by generating cDNA libraries, such as the human brain cDNA library (hEx1). We have made this set non-redundant, containing over 10,000 different human proteins. These proteins have been arrayed on membranes and on glass slides (protein chips). Recent results of screening these human protein arrays with antibodies and peptides, and sera from patients with autoimmune disease will be presented. Recent testing of different surfaces (glass, plastic, coated surfaces) to optimize the protein and antibody chip microscope slide format will be described.

Recommended articles

Cahill, D.J. and Nordhoff, E. (2003).
Protein Arrays and Their Role in Proteomics.
Adv Biochem Engin/Biotechnol 83, 177–187.

Lueking, A., Possling, A., Huber, O., Beveridge, A., Horn, M., Eickhoff, H., Schuchardt, J., Lehrach, H., and Cahill, D.J. (2003).
A Nonredundant Human Protein Chip for Antibody Screening and Serum Profiling.
Molecular & Cellular Proteomics 2, 1342-1349. Available Online