DMMC Course MODEL SYSTEMS IN BIOMEDICAL RESEARCH: SCIENTIFIC BREAKTHROUGHS

1145-1300 Thursday 23 March 2006. UCD Conway Institute Seminar Room 2

Simian virus 40 (SV40): DNA replication
Technical Spotlight: restriction endonucleases
Dr Rosemary Clyne (UCD Conway Institute of Biomolecular and Biomedical Research)

Defining the restriction enzyme map of the simian virus 40 (SV40) genome was a major step toward identifying the functional domains of the viral DNA, including the origin of DNA replication. Identification of the DNA replication origin subsequently permitted the development of a cell-free system that could replicate exogenous viral DNA templates in vitro. The development of this DNA replication system facilitated the purification and characterisation of cellular replication proteins and provided new insight into the mechanisms of DNA synthesis in all eukaryotes.

Clyne Lab web page: http://www.ucd.ie/sbbs/staff/clyne_rosemary/

Resources

Reference: Li, J.J. and Kelly, T.J. (1984). Simian virus 40 DNA replication in vitro. Proc. Natl. Acad. Sci., USA. 81, 6973-6977. PubMed Entry

Web Link: The Nobel Prize in Physiology or Medicine 1978, "for the discovery of restriction enzymes and their application to problems of molecular genetics"